Journal: Cells
Article Title: The Nephrotoxin Puromycin Aminonucleoside Induces Injury in Kidney Organoids Differentiated from Induced Pluripotent Stem Cells
doi: 10.3390/cells11040635
Figure Lengend Snippet: Inflammation-associated gene expression and cytokine secretion in untreated and PAN-treated kidney organoids. ( a ) Pearson heatmap of the expression of immune-related genes in control and PAN treatment. ( b ) Expression of IL-6 and IL-8 is elevated in PAN-induced kidney organoids as measured by qRT-PCR. Error bars depict standard error. ( c ) Cluster dendrogram with the technical duplicates of untreated kidney organoids clustering together and the PAN-treated kidney organoids cluster separately. ( d ) Cytokine array data comparing expression between untreated control and PAN treatment. ( e ) Metascape-generated heatmap comparing UMK1_PAN and UMK1_con included inflammation- and immune-response-related GOs. Subjected gene-sets are based on the Venn analysis in . ( f ) Bar graph of non-redundant enrichment clusters after PAN treatment.
Article Snippet: Untreated organoids and organoids treated with PAN, which had been subjected to analysis with the kidney cytokine assay Human Kidney Biomarker Array Kit (R&D Systems) and scanned, were image-analysed with ImageJ [ ].
Techniques: Gene Expression, Expressing, Control, Quantitative RT-PCR, Generated